Prisoners of war were subjected to vivisection without anesthesia

Prisoners of war were subjected to vivisection without anesthesia. Vivisections were performed on prisoners after infecting them with various diseases. Scientists performed invasive surgery on prisoners, removing organs to study the effects of disease on the human body. These were conducted while the patients were alive because it was feared that the decomposition process would affect the results. The infected and vivisected prisoners included men, women, children, and infants.

Prisoners had limbs amputated in order to study blood loss. Those limbs that were removed were sometimes re-attached to the opposite sides of the body. Some prisoners' limbs were frozen and amputated, while others had limbs frozen then thawed to study the effects of the resultant untreated gangrene and rotting.

Some prisoners had their stomachs surgically removed and the esophagus reattached to the intestines. Parts of the brain, lungs, liver, etc. were removed from some prisoners.

In 2007, the Japanese army surgeon Ken Yuasa testified to the Japan Times that, "I was afraid during my first vivisection, but the second time around, it was much easier. By the third time, I was willing to do it." He believes at least 1,000 people, including surgeons, were involved in vivisections over mainland China

Prisoners were injected with inoculations of disease, disguised as vaccinations, to study their effects. To study the effects of untreated venereal diseases, male and female prisoners were deliberately infected, often by rape, with syphilis and gonorrhea, then studied.

Plague fleas, infected clothing, and infected supplies encased in bombs were dropped on various targets. The resulting cholera, anthrax, and plague were estimated to have killed around and possibly more than 400,000 Chinese civilians. Tularemia was tested on Chinese civilians.

Unit 731 and its affiliated units (Unit 1644 and Unit 100 among others) were involved in research, development, and experimental deployment of epidemic-creating biowarfare weapons in assaults against the Chinese populace (both civilian and military) throughout World War II. Plague-infested fleas, bred in the laboratories of Unit 731 and Unit 1644, were spread by low-flying airplanes upon Chinese cities, coastal Ningbo in 1940, and Changde, Hunan Province, in 1941. This military aerial spraying killed thousands of people with bubonic plague epidemics.

Human targets were used to test grenades positioned at various distances and in different positions. Flame throwers were tested on humans. Humans were tied to stakes and used as targets to test germ-releasing bombs, chemical weapons, and explosives.

In other tests, subjects were deprived of food and water to determine the length of time until death; placed into high-pressure chambers until death; experimented upon to determine the relationship between temperature, burns, and human survival; placed into centrifuges and spun until death; injected with animal blood; exposed to lethal doses of x-rays; subjected to various chemical weapons inside gas chambers; injected with sea water to determine if it could be a substitute for saline; and burned or prematurely buried alive.

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